| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ryan, Katrina A (12)
|
ADSSCY |
32.94 |
32.94 |
0.00 |
| 2 |
Stark, Nikki T (11)
|
ADSARY |
34.85 |
33.19 |
-1.66 |
| 3 |
Chen, Min-syun (12)
|
ADSARY |
35.44 |
36.25 |
0.81 |
| 4 |
Rosenbaum, Jillian (12)
|
ADGLEN |
36.94 |
37.46 |
0.52 |
| 5 |
Trahan, Ella L (11)
|
ADSARY |
38.86 |
37.50 |
-1.36 |
| 6 |
Mitchell, Maris C (12)
|
ADDACC |
41.00 |
37.86 |
-3.14 |
| 7 |
Kuo, Lindsay R (11)
|
ADSSCY |
39.01 |
38.33 |
-0.68 |
| 8 |
Kreig, Hannah (12)
|
ADGLEN |
37.58 |
38.54 |
0.96 |
| 9 |
Mohamed, Amber H (11)
|
ADSSCY |
37.40 |
39.19 |
1.79 |
| 10 |
Trautner, Magdalene Anne B (11)
|
ADSARY |
42.42 |
40.01 |
-2.41 |
| 11 |
Bodien, Julia M (11)
|
ADSSCY |
38.09 |
40.30 |
2.21 |
| 12 |
Zebrowski, Olivia G (12)
|
ADSARY |
42.28 |
40.67 |
-1.61 |
| 13 |
Mienaltowski, Allison E (11)
|
ADDACC |
42.36 |
42.33 |
-0.03 |
| 14 |
Borski, Hannah P (12)
|
ADDACC |
45.51 |
43.65 |
-1.86 |
| 15 |
Taormina, Linaya E (12)
|
ADSSCY |
|
44.36 |
- |
| 16 |
Smith, Dannah J (11)
|
ADDACC |
1:00.58 |
47.42 |
-13.16 |
| 17 |
Krein, Alanna (11)
|
ADGLEN |
57.97 |
54.21 |
-3.76 |